DynamicOrgan® O2-Sensor Kit
The DynamicOrgan® O2-Sensor Kit, developed in collaboration with PyroScience allows for real-time, contact-less, non-invasive detection of oxygen consumption inside an organ model such as lung, gut or liver-on-chip.
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How the oxygen sensor technology works
How to measure oxygen levels in an organ-on-chip model
The heart of DynamicOrgan® O2-Sensor Kit is the 2-channel biochip (BC002) with oxygen sensor spots from PyroScience.
The optical sensors from PyroScience are biocompatible and therefore ideal for sensitive biological methods, such as organ-on-chip. The oxygen meter (FirePlate) excites the oxygen sensitive indicator with its unique REDFLASH technology and detects the oxygen dependent luminescence emission. The higher the oxygen content, the more quenching of the luminescent signal is taking place, allowing for a precise calculation of the oxygen content in the channel of the biochip.
Three sensor spots are incoperated into our biochip, enabling oxygen measurement in the top and bottom channel.
The top channel features a single sensor spot in the center of the top foil for general oxygen level measurement inside the channel. The bottom channel includes two sensor spots on the bottom foil: one positioned at the inlet and another at the outlet. This enables the calculation of oxygen consumption by the cells incorporated in the bottom channel.
Contactless & real-time read-out of oxygen levels
The PyroScience Fireplate readout unit with its biochip holding frame is placed below the biochip for contactless read-out of oxygen sensor spots
This measurement of oxygen levels inside the biochip, without direct intervention in the medium, is a particular advantage of the system. It allows real-time recording of changes in oxygen and its consumption by cells without compromising the integrity of the tissues or organ model.
Additional features of the O2-Sensor Kit & System
Sterilization
The sensor materials are robust against various sterilization methods, including 70% ethanol, ethylene oxide, and plasma irradiation. This makes them easier to use in regulated and sterile laboratory environments, such as incubators.
REDFLASH technology
The REDFLASH technology uses an oxygen-sensitive indicator excitable with orange-red light (610–630 nm) and emitting in the near infrared (760–790 nm). It offers fast response times and low cross-sensitivity. The red excitation minimizes autofluorescence and stress in biological systems.
Why choose the O2-Sensor Kit?
O2-Sensor Kit features
- Oxygen levels for complete organ model
- Direct measurement of oxygen consumption under perfusion
- Compatible with sterilization
- Biocompatible material
- Contact-less readout without interference of the tissue/cells
- Robust read-outs with the REDFLASH technology
- Real-time measurement
- No fixed measurement timepoints
- Environmental control
- Time saving measurement method
Compatible models
Oxygen Sensors are currently available for our 2-channel biochip (BC002) only. Any organ models previously developed on this biochip can be transferred to the O2-Sensor biochip.
Examples of organs-on-chips developed on the 2-channel biochip:
What’s included in the O2-Sensor Kit?
The Kit is available in three different size options:
DynamicOrgan® O2-Sensor Kit – S
10x Biochips (BC002 with O2 Sensors)
20x 2-Stop Tubing
40x Connectors (Adapter)
20x Reservoirs
DynamicOrgan® O2-Sensor – M
25x Biochips (BC002 with O2 Sensors)
50x 2-Stop Tubing
100x Connectors (Adapter)
50x Reservoirs
DynamicOrgan® O2-Sensor Kit – L
50x Biochips (BC002 with O2 Sensors)
100x 2-Stop Tubing
200x Connectors (Adapter)
100x Reservoirs
What do I need to get started?
In addition to the kit, you will require a peristaltic pump and the measurement device for your oxygen sensor read-outs (FirePlate from PyroScience) plus a holding frame suitable to host 3 biochips in parallel. See below for the options available, depending on whether you are already a user of our system or not.
If you are already a user of Dynamic42 biochips:
DynamicOrgan O2-Sensor System Pro – includes the kit size of your choice and the PyroScience Fireplate readout unit plus the biochip holding frame.
If you haven’t used our products before:
DynamicOrgan O2-Sensor System Beginner includes the kit size of your choice, a pressure pump and the PyroScience Fireplate readout unit plus the biochip holding frame.
FAQ
What is the DynamicOrgan® O2-Sensor Kit and how does it work?
The DynamicOrgan® O2-Sensor Kit is a tool designed for measuring oxygen levels in organ-on-chip models. It works by utilizing optical oxygen sensor spots from PyroScience that enable non-invasive oxygen measurement.
How does the PyroScience technology enable non-invasive oxygen measurement in organ-on-chip models?
The FirePlate oxygen meter uses REDFLASH technology to detect oxygen-dependent luminescence emission, allowing for precise oxygen content calculation in biochip channels without disturbing the sample.
What are the benefits of using the DynamicOrgan® O2-Sensor Kit for organ research?
The benefits of using the DynamicOrgan® O2-Sensor Kit include direct measurement of Oxygen levels under perfusion using REDFLASH technology. This method is compatible with sterilization, biocompatible, and provides robust, real-time measurements without interference.
Can the DynamicOrgan® O2-Sensor Kit be used with different types of organ-on-chip models?
Yes, the DynamicOrgan® O2-Sensor Kit can be used with different types of organ-on-chip models, offering flexibility in experimental design.
What sterilization methods are compatible with the sensor materials in the DynamicOrgan® O2-Sensor Kit?
The sensor materials are resilient against various sterilization methods, such as 70% ethanol, ethylene oxide, and plasma irradiation.
What additional equipment is required to use the DynamicOrgan® O2-Sensor Kit?
Besides the kit size of your choice, a pressure pump and the PyroScience Fireplate readout unit plus the biochip holding frame are needed. Should you already have a preexisting Fireplate or pump system only the holding frame specifically designed to hold 3 biochips will be needed in addition to the kit.
How can I measure oxygen consumption in the biochip?
The bottom channel of the O2-Sensor biochips is equipped with a sensor spot at the inlet as well as the outlet. This enables the calculation of oxygen consumption by the tissue incorporated in the bottom channel.
