DynamicOrgan® Spheroid Kit
This kit contains all consumables needed to combine a barrier with spheroids or organoids in one multilayer organ model. This could be used to combine a vasculature with a tumor or other spheroids of your choice.
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The biochip – the heart of the organ model
The Spheroid Kit hosts our BC003, a biochip containing two membranes (1x porous, 1x microcavity) dividing the culture chamber into a top, middle and bottom channel accessible via ports. The middle channel with the microcavity membrane can host up to 25 spheroids or organoids.
Cell suspensions and coatings can be introduced individually into each channel, enabling the creation of cell layers and complex tissues on both sides of the membrane.
The separation of the channels allows nourishing of the tissues and spheroids with media and individual flow rates per channel (or no flow at all).
Additionally, circulating cell populations can be introduced. Matching reservoirs facilitate perfusion and ensure nutritional supply up to three days without medium exchange.
BC003 Datasheet
When to use the Spheroid Kit?
Spheroid-on-chip
Organoid-on-chip
Easy in-chip imaging of Spheroids
Easy sampling of Spheroid
The Spheroid Kit is particularly suitable if you need your spheroids or organoids to be aligned in a single layer and not randomly distributed inside the channel.
If you are unsure if the 3-Channel or Spheroid Kit might be best suited for your application, we suggest speaking to one of our scientists.
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Spheroid Kit Product Information
What’s included in the Spheroid Kit?
The kit is available in three different size options:
DynamicOrgan® Spheroid Kit – S
10x Biochip BC003
20x 2-Stop Tubing
25x Small Reservoir
50x Luer Adapter
135x Luer Plug
DynamicOrgan® Spheroid Kit – M
25x Biochip BC003
50x 2-Stop Tubing
25x Small Reservoir
50x Luer Adapter
135x Luer Plug
DynamicOrgan® Spheroid Kit – L
50x Biochip BC003
100x 2-Stop Tubing
25x Small Reservoir
50x Luer Adapter
135x Luer Plug
Additionally, all kits are available with different membranes (0.4, 3, 5 or 8µm) and microscope cover glass at the bottom.
What do I need to get started?
In addition to the kit, you will require a peristaltic pump. If you don’t have one, you can order a DynamicOrgan® System and will receive a peristaltic pump in addition to the Spheroid Kit.
Additional material for advanced applications
All kits are intended for one-channel perfusion with small reservoirs. Below you can find a selection of extension kits if double perfusion (such as for intestinal models) or large reservoirs are required.
DynamicOrgan® Double Perfusion Set – S
20x 2-Stop Tubing
25x Small Reservoirs
50x Connectors (Adapter)
DynamicOrgan® Double Perfusion Set – M
50x 2-Stop Tubing
25x Small Reservoirs
50x Connectors (Adapter)
DynamicOrgan® Double Perfusion Set – L
100x 2-Stop Tubing
25x Small Reservoirs
50x Connectors (Adapter)
Large Volume Set
25x Large Reservoir + Adapter
DynamicOrgan® Large Volume Double Perfusion Set – S
20x 2-Stop Tubing
25x Large Reservoirs + Adapter
DynamicOrgan® Large Volume Double Perfusion Set – M
50x 2-Stop Tubing
25x Large Reservoirs + Adapter
DynamicOrgan® Large Volume Double Perfusion Set – L
100x 2-Stop Tubing
25x Large Reservoirs + Adapter
FAQ
What is a spheroid-on-chip model?
Spheroid-on-chip combines advanced 3D cell culture models like spheroids and organoids with microfluidic on-chip devices.
What advantage does spheroid-on-chip offer compared to using spheroids in static culture?
Traditional methods of spheroid formation, such as hanging drop cultures or microwell plates, have been instrumental in the development of 3D cell culture. However, they have significant limitations. The static nature of these culture environments can lead to rapid depletion of oxygen and nutrients, as well as accumulation of waste products, which adversely affect spheroid viability and the reliability of experimental results. In contrast, microfluidic spheroid-on-chip models offer a more sophisticated approach to spheroid culture. These biochips allow for precise control over the microenvironment, including regulation of chemical gradients, pressure, and shear stress. This level of control is crucial for mimicking the dynamic conditions of in vivo tissues.
How is spheroid-on-chip in our Spheroid Kit different to using spheroids in our 3-Channel Kit?
The Spheroid Kit includes the BC003 biochip hosting one flat and one micro-cavity membrane. This allows for arrangement of spheroid into a single layer and for easier imaging and sampling. The 3-Channel Kit includes the BC003 biochip with 2 flat membranes. Here, spheroids are inserted together with a gel matrix which allows the spheroids to arrange in a random fashion closer to the in vivo situation of a tumor.
What is the maximum size of spheroids that can be introduced in the biochip?
Due to the channel size, spheroid diameter is limited to a maximum of 800µm.
Can Spheroids be recovered during or after the experiment?
With the spheroid kit spheroids or organoids are loaded without a gel matrix onto the biochip allowing for them to be recovered more easily. Spheroids can either be recovered from the channel directly or through cutting of the membrane after the experiment has concluded.
How can I acquire the spheroid kit?
Please contact us via the form provided for ordering options and product availability.
Can I commission a custom study using spheroid-on-chip technology?
Yes, our service team supports custom preclinical research projects using spheroid-on-chip models. Learn more about our study design and CRO services at dynamic42.com/services
Is training available for using the spheroid-on-chip in the lab?
Our Dynamic42 Academy offers hands-on and virtual training courses to help you confidently apply organ-on-chip models in your lab. We currently offer basic training for our barrier models and advanced training for cancer-on-chip using our 3-channel kit. Explore available formats here: dynamic42.com/academy
