DynamicOrgan® TEER-Sensor Kit
The DynamicOrgan® TEER-Sensor Kit allows for real-time, non-invasive monitoring of barrier integrity within an organ-on-chip model.
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How the TEER sensor technology works
How to measure TEER in an organ-on-chip model
The heart of the DynamicOrgan® TEER-Sensor Kit is the 2-channel biochip (BC002) with fixed plane gold-electrodes.
The BC002 consists of a perfused top and bottom channel separated by a porous membrane. The biochip is sealed by a bonding foil on the top and bottom side. The gold electrodes are spotted on the insides of the bonding foils, allowing an alternating current to flow between the two electrodes at the top and bottom position.
The Trans-Epithelial/Trans-Endothelial Electrical Resistance (TEER) between the two electrodes is detected by a measurement device.
Resistance arises from the tissue layers between the electrodes, with higher resistance indicating a confluent and intact tissue barrier.
Non-invasive, robust TEER measurement
The direct integration of immobilised electrodes within the biochip, in close proximity to the tissue, reduces signal noise caused by electrode movement, as observed in other TEER measurement methods like the common “chopstick” method.
This also decreases the risk of cell damage, as the electrodes are already immobilised and don’t need to be placed in the culture system.
The electrode signals are transmitted to the tighTEER readout unit via an integrated circuit board. This board connects to the tighTEER readout unit through electric wires that can be easily attached to the chip’s circuit board in a plug-and-play configuration. The tighTEER is capable of simultaneously monitoring TEER values from three independent biochips (a total of six biochip cavities). The recorded TEER data are transferred wirelessly to an external computer enabling real-time monitoring and visualization of the measurements at user-defined time intervals.
Why choose the TEER-Sensor Kit?
TEER Kit features
✅ Real-time, and long-term TEER measurement
Existing methods are mostly endpoint based
✅ Compatibility with on-chip light microscopic imaging
TEER electrodes are semi-transparent
✅ Neglectable noise from electrode motion + non-invasive measurement method
Our electrodes are fixed to the biochip in contrast to existing technologies
✅ Quick set up & easy handling also for inexperienced users
All you need to do is connect the biochip via a cable to the tighTEER readout unit.
✅ TEER is measured directly inside the incubator and transmitted wirelessly to a computer
TEER measurements are temperature sensitive. Recording the values inside the incubator ensures robust and reproducible measurements between experiments and replicates.
Compatible models
TEER Sensors are currently available on our 2-channel biochip (BC002) only. Below are examples of organ models previously developed on the 2-channel biochip:
What’s included in the TEER Sensor Kit?
The Kit is available in three different size options:
DynamicOrgan® TEER-Sensor Kit – S
10x Biochips (BC002 with TEER Sensors)
50x 2-Stop Tubing
25x Small Reservoir
50x Luer Adapter
90x Luer Plug
24x Mini Luer Plug
DynamicOrgan® TEER-Sensor – M
25x Biochips (BC002 with TEER Sensors)
100x 2-Stop Tubing
25x Small Reservoir
50x Luer Adapter
90x Luer Plug
24x Mini Luer Plug
What do I need to get started?
In addition to the kit, you will require a peristaltic pump and the measurement device for your TEER sensor read-outs. See below the options available depending on if you are already a user of our system or not.
If you are already a user of Dynamic42 biochips:
DynamicOrgan TEER-Sensor System Pro – includes the kit site of your choice and the tighTEER T-31 readout unit.
If you haven’t used our products before:
DynamicOrgan TEER-Sensor System Beginner – includes the kit site of your choice, a peristaltic pump and the tighTEER readout unit.
FAQ
What is the DynamicOrgan® TEER-Sensor Kit used for?
The DynamicOrgan® TEER-Sensor Kit is used for real-time, non-invasive monitoring of barrier integrity within an organ-on-chip model.
How does the TEER-Sensor Kit work?
The kit uses a 2-channel biochip (BC002) with fixed plane gold-electrodes to measure Trans-Epithelial/Trans-Endothelial Electrical Resistance (TEER). The biochip consists of a perfused top and bottom channel separated by a porous membrane, allowing an alternating current to flow between the electrodes to detect resistance.
What are the advantages of using the DynamicOrgan® TEER-Sensor Kit?
The kit offers reliable, real-time, and long-term TEER measurement, fast and accurate results, compatibility with on-chip live-cell brightfield imaging, negligible noise from electrode motion, quick setup, easy handling, biocompatibility, non-invasiveness, and low compound adsorption.
Can the DynamicOrgan® TEER-Sensor Kit be used with different types of organ-on-chip models?
Yes, the TEER-Sensor biochip can be used to establish different types of organ-on-chip models. The basis of this biochip forms our BC002 2-channel biochip for which recently lung, intestine, gut and vascular models have been established.
Can the biochip inside DynamicOrgan® TEER-Sensor Kit be sterilized?
The biochip itself and the integrated sensor materials are resilient against sterilization with 70% ethanol. Since biochips are delivered unsterile within the kit, sterilization is mandatory before seeding of cells.
What additional equipment is needed to use the TEER-Sensor Kit?
In addition to the kit, a peristaltic pump and the measurement device for TEER sensor read-outs are required.
Do you service offerings include TEER measurement?
Yes, TEER can be requested as a read-out for our custom preclinical research projects. Learn more about our study design and CRO services.
