The development of an active substance from its discovery to approval is very time-consuming and costly. Withdrawing a drug in the human clinical phase as well as after approval due to unforeseen side effects is a waste of resources that you do not need and that your stakeholders want to avoid. Therefore, a reliable in vitro technology that provides you with information on whether your compound will be tolerable or toxic is of high economic and ethical interest. The human in vitro organ models created and offered by Dynamic42 GmbH serve this need. They are composed of a blood vessel structure on the one hand and an organ-specific tissue on the other. The perfusion of the blood vessel side allows the formation of an in vivo-like microenvironment. The integration of tissue-resident immune cells into our models enables us to detect side effects that cannot be detected by conventional systems. The services offered by Dynamic42 use human in vivo-like organ models to help you answer the question of whether your drug is toxic or tolerable. It helps you decide whether to continue with preclinical studies or to stop drug development before animal testing.

If you have specific requirements, please do not hesitate to contact us. Our experienced scientists will tailor your project for you to test your drug candidates in such a way that you receive the required information cost-effectively and promptly.

Cell type-specific assays

We offer a range of cell type-specific assays for the pre-selection of drug candidates. Mono-cell cultures as well as co-cultures are cultivated in 2D or 3D, static or dynamic according to your requirements.

We have established the following assays:

Monocytes/Macrophages

  • Recording assays: Phagocytosis,
  • cytokine release
  • Adhesion/Patrolling/Transmigration (in co-culture with endothelial cells)

Hepatocytes

  • Recording assays on transporter proteins
  • excretion assays
  • metabolisation assays
  • Vitality check based on the release of ALAT/ASAT, LDH,

Endothelial cells

  • Adhesion assays with immune cells
  • Activation (in combination with macrophages)
  • Barrier Function Assays
  • Interaction studies (e.g. nanoparticles)

Organ models

MRP-2 expression (orange) indicates the presence and expression of bile canaliculi in the liver sinusoid model.

Liver sinusoid model

We offer the following analyses for this model:

  • Immunofluorescence: Marker expression, uptake studies (Mø, LSEC, hepatocytes)
  • Clinical parameters for vitality and liver function: LDH, ASAT/ALAT, urea, albumin, …
  • cytokine profile
  • Excretion: CDFDA assay
  • Metabolisation: CYP3A4, CYP2C9, CYP2C19
  • Perfusion of immune cells
  • oxygen consumption
  • Liver zoning due to oxygen gradients
  • Flow cytometry: recording studies, marker expression, analysis of specific cell populations
  • qRT-PCR

Expression of the adherence junction protein E-cadherin (orange) and surfactant protein A expression (red) on lung epithelial cells in the alveolar model.

Alveolus

We offer the following analyses for this model:

  • Immunofluorescence: marker expression, uptake studies, pathogens
  • Clinical parameters: LDH, lactate, glucose
  • cytokine profile
  • Perfusion of immune cells
  • Resistance measurements (TEER) and permeability assays
  • Flow cytometry: recording studies, marker expression, analysis of specific cell populations
  • qRT-PCR
  • Surfactant Production

Mucin 2 secretion (orange) by enterocytes. Epithelial barrier integrity is indicated by the presence of E-cadherin (red) in the intestinal model.

Intestine

We offer the following analyses for this model:

  • Immunofluorescence: marker expression, uptake studies, pathogens
  • Clinical parameters: LDH, lactate, glucose
  • cytokine profile
  • Perfusion of immune cells
  • Resistance measurements (TEER) and permeability assays
  • Flow cytometry: recording studies, marker expression, analysis of specific cell populations
  • qRT-PCR
  • Analyses of the Mucus
  • Oxygen consumption and gradients
  • Villus and crypts formation

Customized solutions

Do you have special questions, wishes and (project) ideas?

Please do not hesitate to contact us. Write to us at info@dynamic42.com or call us at +49 (0)3641 508 101!