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The de­vel­op­ment of an ac­tive sub­stance from its dis­cov­ery to ap­proval is very time-con­sum­ing and cost­ly. With­draw­ing a drug in the hu­man clin­i­cal phase as well as af­ter ap­proval due to un­fore­seen side ef­fects is a waste of re­sources that you do not need and that your stake­hold­ers want to avoid.

There­fore, a re­li­able in vit­ro tech­nol­ogy that pro­vides you with in­for­ma­tion on whether your com­pound will be tol­er­a­ble or toxic is of high eco­nomic and eth­i­cal in­ter­est. The hu­man in vit­ro mi­cro­phys­i­o­log­i­cal mod­els cre­ated and of­fered by Dy­nam­ic42 GmbH can ad­dress this need. They are com­posed of a blood ves­sel struc­ture on the one hand and an or­gan-spe­cific tis­sue on the oth­er. The per­fu­sion of the blood ves­sel com­part­ment al­lows the for­ma­tion of an in vivo-like mi­croen­vi­ron­ment. Our abil­ity to per­fuse and in­te­grate tis­sue-res­i­dent im­mune cells into our mod­els en­ables us to de­tect side ef­fects that can­not be de­tected by con­ven­tional sys­tems.

If you have spe­cific re­quire­ments, please do not hes­i­tate to contact us. Our ex­pe­ri­enced sci­en­tists will tai­lor your project for you to ad­dress your sci­en­tific ques­tions in such a way that you re­ceive the re­quired in­for­ma­tion cost-ef­fec­tively and prompt­ly.

Cell Type Specific Assays

We of­fer a range of cell type-spe­cific as­says for the pre-e­val­u­a­tion of cell cul­ture set­tings and com­pounds. Mono-cell cul­tures as well as co-cul­tures are cul­ti­vated in 2D or 3D, sta­tic or dy­namic ac­cord­ing to your re­quire­ments.

We have es­tab­lished the fol­low­ing as­says:

Immune Cells

  • Up­take as­says: Phago­cy­to­sis
  • Cy­tokine re­lease
  • Ad­he­sion/ Pa­trolling/ Trans­mi­gra­tion (in co-cul­ture with en­dothe­lial cells)

Hepatocytes

  • Ac­tiv­ity as­says on trans­porter pro­teins
  • En­zyme ac­tiv­ity as­says
  • Ex­cre­tion as­says
  • Metaboli­sa­tion as­says
  • Vi­tal­ity check based on the re­lease of ALAT/ASAT, LDH
  • ROS For­ma­tion

Endothelial Cells

  • Ad­he­sion as­says with im­mune cells
  • En­zyme ac­tiv­ity as­says
  • Ac­ti­va­tion (in com­bi­na­tion with macrophages)
  • Bar­rier Func­tion As­says
  • In­ter­ac­tion stud­ies (e.g. nanopar­ti­cles)

Enterocytes

  • Trans­porter ac­tiv­ity as­says
  • En­zyme ac­tiv­ity as­says
  • Bar­rier func­tion as­says
  • Metaboli­sa­tion as­says
  • Up­take as­says
  • Vi­tal­ity check based on the re­lease of LDH.

Model Specific Analyses

For each of our or­gan mod­els we of­fer ser­vices spe­cific to that mod­el.

Liver Sinusoid Model

  • Im­muno­flu­o­res­cence: Marker ex­pres­sion, up­take stud­ies (Mø, LSEC, he­pa­to­cytes)
  • Clin­i­cal pa­ra­me­ters for vi­tal­ity and liver func­tion: LDH, ASAT/ALAT, urea, al­bu­min, glu­cose, lac­tate
  • Cy­tokine pro­file
  • Ex­cre­tion: CDFDA as­say
  • Metaboli­sa­tion: CYP3A4, CYP2C9, CYP2C19
  • Per­fu­sion of im­mune cells
  • Oxy­gen con­sump­tion
  • Liver zon­ing due to oxy­gen gra­di­ents
  • Flow cy­tom­e­try: record­ing stud­ies, marker ex­pres­sion, analy­sis of spe­cific cell pop­u­la­tions
  • qRT-PCR

Alveolus

  • Im­muno­flu­o­res­cence: marker ex­pres­sion, up­take stud­ies, pathogens
  • Clin­i­cal pa­ra­me­ters: LDH, lac­tate, glu­cose
  • Cy­tokine pro­file
  • Per­fu­sion of im­mune cells
  • Re­sis­tance mea­sure­ments (TEER) and per­me­abil­ity as­says
  • Flow cy­tom­e­try: record­ing stud­ies, marker ex­pres­sion, analy­sis of spe­cific cell pop­u­la­tions
  • qRT-PCR
  • Sur­fac­tant Pro­duc­tion

Intestine

  • Im­muno­flu­o­res­cence: marker ex­pres­sion, up­take stud­ies, pathogens
  • Clin­i­cal pa­ra­me­ters: LDH, lac­tate, glu­cose
  • Cy­tokine pro­file
  • Per­fu­sion of im­mune cells
  • Re­sis­tance mea­sure­ments (TEER) and per­me­abil­ity as­says
  • Flow cy­tom­e­try: record­ing stud­ies, marker ex­pres­sion, analy­sis of spe­cific cell pop­u­la­tions
  • qRT-PCR
  • Analy­ses of the Mu­cus
  • Oxy­gen con­sump­tion and gra­di­ents
  • Vil­lus and crypts for­ma­tion

Customised Solutions

Do you have spe­cial ques­tions, wishes or ideas?

Please do not hes­i­tate to contact us.