Endothelial cells line blood vessels to form the natural barrier between blood and tissue. We included Human Pancreatic Microvascular Endothelial Cells (HPaMVECs) into the perfused upper channel of the CHIP, to mimic a vascular linkage of spheroids in the middle chamber.
Spheroids are used to model a plethora of healthy or malignant tissue types. They are more reliant than 2D cultures in predicting in vivo tissue functions. In combination with the Dynamic42 biochip, spheroids become then a popular system for dynamic drug screening, tissue engineering and basic science.
Our immunocompetent spheroid-on-chip cultures can be set up with a vasculature for indirect perfusion providing fresh nutrients, an oxygen gradient and ensuring removal of waste products, while avoiding shear stress on the spheroids.
Model set up
The Dynamic42 Spheroid-on-Chip comprises 3 compartments: a vascular for perfusion, a microcavity channel able to host 25 spheroid or organoids and a third channel which can be used for spheroid feeding and sampling of spheroid conditioned supernatant without disturbing the spheroids themselves. The vasculature is formed by an endothelial lining and tissue-resident macrophages.
The Dynamic42 Spheroid-on-Chip can be operated with various cell sources. Please get in contact for further details.
Endothelial Cell
Spheroid
Two models can be operated in parallel on one Dynamic42 biochip.
Features
/ Up to 2 x 25 spheroids on one biochip.
/ Immobilization of spheroids, enabling repetitive analysis of the same spheroid over time
/ Endothelial channel to recreate the in vivo vascular barrier for nutrition, oxygen, immune cells and drugs
/ Indirect perfusion through vasculature to avoid shear stress on spheroids
/ Spheroid feeding and sampling of spheroid conditioned supernatant without disturbance of spheroids
/ Live imaging of spheroids, e.g. monitoring Mitochondria membrane potential during treatment
/ All spheroids are easily recovered through cutting of the biochip after the experiment for end point analysis
/ Usage of magnetic bead allows for sampling of spheroids during an experiment.
/ Independent perfusion of upper and lower channel allows complex model setups
Applications
/ Drug development
/ Uptake / Transport studies
/ Disease modelling (such as Tumor models)
/ Complex multi-layer co-culture
Characteristics
/ Complex multilayer co-culture
/ Immune-ready
/ Vascularization
/ Perfusion
/ Extracellular Matrix compatible
/ Sampling of spheroids during the experiment
Related Products
/ Three-channel biochip
/ Including one channel with microcavities (⌀ 800μm)
/ Cultivation of up to 2×25 spheroids in parallel
/ Two culture chambers per biochip
/ Low-adsorption material biochip
Build human in vitro disease and infection models with the DynamicOrgan System. A system that includes pumps, biochips, consumables and the freedom to keep using standard laboratory equipment. A dynamic system that will help you get greater and more meaningful insights into human biology swiftly and without capital spending.
