Loading of spheroids in the microcavities inside the BC003 biochip.
Spheroids and organoids are used to model a plethora of healthy or malignant tissue types. They are more reliant than 2D cultures in predicting in vivo tissue functions. In combination with the DynamicOrgan® Spheroid Kit, spheroids become then a popular system for dynamic drug screening, tissue engineering and basic science.
Our immunocompetent spheroid- or organoid-on-a-chip models can be set up with a vasculature for indirect perfusion providing fresh nutrients, an oxygen gradient and ensuring removal of waste products, while avoiding shear stress on the spheroids.
Spheroid model set up
The Dynamic42 Spheroid Kit hosts our BC003 biochip which contains 3 channels for cell culture: a vascular for perfusion, a channel with a microcavity membrane able to host 25 spheroid or organoids and a third channel which can be used for spheroid feeding and sampling of spheroid conditioned supernatant without disturbing the spheroids themselves. The vasculature is formed by an endothelial lining and can include tissue-resident macrophages.
The DynamicOrgan® Spheroid Kit can be operated with various cell sources. Please get in contact for further details.
Endothelial Cell
Spheroid
Two models can be operated in parallel on one Dynamic42 biochip.
Features
/ Up to 2 x 25 spheroids on one biochip.
/ Immobilization of spheroids, enabling repetitive analysis of the same spheroid over time
/ Endothelial channel to recreate the in vivo vascular barrier for nutrition, oxygen, immune cells and drugs
/ Indirect perfusion through vasculature to avoid shear stress on spheroids
/ Spheroid feeding and sampling of spheroid conditioned supernatant without disturbance of spheroids
/ Live imaging of spheroids, e.g. monitoring Mitochondria membrane potential during treatment
/ All spheroids are easily recovered through cutting of the biochip after the experiment for end point analysis
/ Usage of magnetic bead allows for sampling of spheroids during an experiment.
/ Independent perfusion of upper and lower channel allows complex model setups
Applications
/ Drug development
/ Uptake / Transport studies
/ Disease modelling (such as Tumor models)
/ Complex multi-layer co-culture
Characteristics
/ Complex multilayer co-culture
/ Immune-ready
/ Vascularization
/ Perfusion
/ Extracellular Matrix compatible
/ Sampling of spheroids during the experiment
Related Products
- Build organ-on-chip models containing a barrier and spheroids
- Kit contains all consumables to combine a barrier with spheroids or organoids in one multilayer organ model
More information
What do I need to get started?
In addition to the kit, you will require a peristaltic pump. If you don’t have one, you can order a DynamicOrgan System and will receive a peristaltic pump in addition to your kit of choice.
