Organ-on-a-Chip Platform

Our hu­man, phys­i­o­log­i­cal cell cul­ture sys­tems are cre­ated in a spe­cially de­vel­oped biochip, made of bio­com­pat­i­ble plas­tic, suit­able for chem­i­cal and tox­i­co­log­i­cal tests. By in­te­grat­ing a porous mem­brane into the biochip, we achieve a spa­tial sep­a­ra­tion of the blood ves­sel and or­gan-spe­cific tis­sues. The prop­er­ties of the mem­brane al­low an ex­change of nu­tri­ents and oxy­gen as well as im­por­tant cell in­ter­ac­tions be­tween the tis­sues (e.g. paracrine sig­nal mol­e­cules; trans­mi­gra­tion).

Cells can be in­di­vid­u­ally in­tro­duced into the biochip and con­tin­u­ously sup­plied with nu­tri­ents and oxy­gen via chan­nels and ex­posed to me­chan­i­cal stim­uli. This is of de­ci­sive im­por­tance for the tis­sue de­vel­op­ment and arrange­ment as well as the func­tional main­te­nance of the tis­sue over weeks.

By in­te­grat­ing sen­sors to mon­i­tor oxy­gen con­sump­tion and bar­rier func­tion, we are able to mon­i­tor tis­sue vi­a­bil­ity and func­tion­al­ity in real time. This en­ables us to de­tect the tox­i­c­ity of ac­tive sub­stances in a very sen­si­tive and timely man­ner.

Applications

  • Preclinical phase of drug development (prior to animal testing)

  • Review of novel therapy options (e.g. uptake, toxicity of nanoparticles)

  • Toxicity determination for newly developed chemicals

  • Cell-based culture systems in basic research

  • Food toxicology

  • Human disease and infection models for drug testing or the investigation of mechanistic questions

Our Products

Microfluidic Chip

The heart of our mi­cro­phys­i­o­log­i­cal cell cul­ture tech­nol­o­gy: Our mi­croflu­idic chip! The chip com­prises two cav­i­ties and is de­signed to op­er­ate two mi­cro­phys­i­o­log­i­cal mod­els in par­al­lel, ei­ther equal ones or dif­fer­ent ones. Fur­ther, the mod­els can be in­ter­con­nect­ed, if de­sired. The chip body is made of bio­com­pat­i­ble non-PDM­S-based syn­thetic ma­te­r­ial and the cav­i­ties are sub­di­vided into an up­per and lower cir­cu­la­tion chan­nel by a thin porous PET mem­brane. The mem­brane is tightly sealed and flat en­abling the cul­ture of tight tis­sue bar­ri­ers and live cell imag­ing. The chan­nels of each cav­ity can be op­er­ated in­di­vid­u­al­ly, thus sup­port­ing com­plex 3D cell cul­ture with unique needs. For more in­for­ma­tion, please contact us.

De­spite our ser­vice of­fers to you, it is pos­si­ble to ac­quire the chips sep­a­rately for your own re­search pro­jects. Along, we of­fer you also a train­ing for op­er­at­ing the chip plat­form.

Liver Sinusoid Model

The liver is the cen­tral meta­bolic or­gan in our body. It is re­spon­si­ble for the me­tab­o­lism of plasma pro­teins, car­bo­hy­drates and lipids and for the degra­da­tion of a large num­ber of drugs and tox­ins and is there­fore of par­tic­u­lar im­por­tance in the de­vel­op­ment of new drugs.

Our hu­man liver si­nu­soid model is based on the anatom­i­cal ba­sic unit of the liv­er, the liver si­nu­soid, in terms of struc­ture, cell com­po­si­tion and cul­ture con­di­tions and can cur­rently be func­tion­ally main­tained over 10 days. Through the in­te­gra­tion of im­mune cells, side ef­fect­s/­tox­i­c­ity of ac­tive sub­stances can be made vis­i­ble at an early stage with this sys­tem.

Alveolus Model

The task of the lung is the ex­change of gas­es, which is made pos­si­ble by a large sur­face area cre­ated by the dis­tal branch­ing of the tra­chea into bronchi, bron­chi­oli and alve­oli. With our hu­man alve­o­lar mod­el, hu­man alve­o­lar ep­ithe­lial and en­dothe­lial cells as well as es­sen­tial com­po­nents of the im­mune sys­tem can be func­tion­ally cul­ti­vated for 21 days. Hereby tox­i­c­i­ties of in­haled sub­stances (e.g. res­pi­ra­tory sen­si­tiz­ers, al­ler­gens, fine dust / diesel) can be test­ed. The model is also suit­able for con­duct­ing in­fec­tion stud­ies and test­ing new ther­a­peu­tics.

Intestine Model

The in­tes­tine is a very im­por­tant part of the di­ges­tive tract. It serves the ab­sorp­tion of nu­tri­ents, the reg­u­la­tion of the wa­ter bal­ance, the for­ma­tion of im­mune cells as well as the pro­duc­tion of hor­mones and mes­sen­ger sub­stances.

Our three-di­men­sional in­testi­nal mod­el, which can be func­tion­ally main­tained over 15 days, forms Vil­lus-like struc­tures con­tain­ing the char­ac­ter­is­tic cell types as well as cen­tral tis­sue-re­sis­tant im­mune cells. This model can be used to in­ves­ti­gate ques­tions re­gard­ing the up­take of ac­tive sub­stances and their trans­port into the blood­stream as well as di­rect toxic ef­fects on the in­testi­nal tis­sue and its bar­rier func­tion.

By com­bin­ing these three hu­man or­gan mod­els, we can also ad­dress and an­a­lyze a wide range of ques­tions re­gard­ing AD­MET (ab­sorp­tion, dis­tri­b­u­tion, me­tab­o­lism, ex­cre­tion, tox­i­col­o­gy) of an ac­tive sub­stance.