DynamicOrgan® TME Kit
The TME Kit creates a biomimetic matrix inside the biochip, empowering researchers to build advanced cancer-on-chip models with a complex tumor microenvironment (TME) and microvasculature.
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How to use the Tumor Microenvironment Kit
The DynamicOrgan® TME Kit is designed for use with our DynamicOrgan® 3-Channel System.
The System will include a peristaltic pump and the DynamicOrgan® 3-Channel Kit featuring advanced 3-channel Biochips.
The TME Kit allows for the embedding of tumor spheroids within a matrix inside the 3-channel biochip, generating a perfusable, microvascularised 3D tumour structure.
Combined with the peristaltic pump included in the System, this setup supports continuous perfusion and thus highly dynamic modeling of the TME.
TME Kit Flyer
Matrix supports 3D co-culture of multiple cell types
- Tumor cells
- Cancer associated fibroblasts
- Immune cells
- Endothelial cells (vasculature)
Applications
- TME interaction
- Immune invasion
- Neoangiogenesis
- Metastasis
- Dynamic drug application
What’s included in the TME Kit?
5 vials Matrix Component A
5 vials Matrix Component B
1 vial Dilution Buffer A
1 vial Dilution Buffer B
2 vials Growth Factor Mix 1
1 vial Growth Factor Mix 2
20x Short Tubing
40x Luer Adaptors
40x Small Reservoir
40x Small Reservoir Scew-on Lid
One TME Kit is enough for 10 biochips (Kit size S)
What do I need to get started?
In addition to the TME Kit, you will require the DynamicOrgan® 3-Channel System, which includes a peristaltic pump and the DynamicOrgan® 3-Channel Kit.
FAQ
What are the benefits of using the DynamicOrgan® TME Kit for cancer research?
• Enables creation of 3D tumor architecture, essential for realistic cancer modeling.
• Mimics gradients of oxygen, nutrients, and metabolites, plus mechanical cues.
• Supports emergence of cancer hallmarks:
o Angiogenesis & metabolic reprogramming: Hypoxia triggers VEGF and glycolytic shifts.
o Immune evasion: Physical barriers and stromal components hinder immune infiltration.
o Sustained proliferative signaling: Cell–cell and cell–matrix interactions enhance growth factor signaling.
• Resistance to cell death: Hypoxic cores and ECM stiffness activate survival pathways.
• Invasion & metastasis: 3D structure enables EMT and migration through ECM.
Where can I learn how to use the tumor microenvironment kit?
In the Dynamic42 Academy, we are providing advanced 3-day courses on cancer-on-chip. These courses will teach you how to utilize the 3-channel biochip and set up your own perfused cancer-on-chip model.
Does the TME Kit work with all cancer types?
The TME-Kit Matrix is designed to work with many tumors but is not adapted for a specific cancer type. It acts as a foundation for cancer spheroids, allowing the tumor to define its own tumor microenvironment.
Can the TME Kit be integrated with other organ-on-chip systems?
The TME Kit size and composition was specifically designed to work with the DynamicOrgan 3-Channel System.
Are there recommended protocols for co-culturing tumor and immune cells in the biochip?
The TME Kit will be provided along with an in-depth protocol that describes the process of introducing the matrix, endothelial cells, and tumor spheroids into the 3-channel biochips.
Are there training materials or video tutorials for using the TME Kit?
The TME Kit will come with a protocol that explains how to introduce the matrix, endothelial cells, and tumor spheroids into the 3-channel biochips. Starting from Q1 2026, we will also provide video tutorials for the TME Kit in our Online Academy.
Is the TME Kit compatible with live-cell imaging or fluorescence microscopy?
Yes. The TME Kit is used in combination with out DynamicOrgan 3-Channel Kit. The biochips included in this kit are in a microscope slide format with a clear viewing window, enabling imaging of the tumor microenvironment. Moreover, tumors can be recovered from the biochip by cutting the membrane, facilitating read-outs such as immunofluorescence staining after the experiment.
How long can 3D tumor cultures be maintained in the TME Kit?
• Vasculature forms in 6–8 days
• Experiments should be performed within ~4 additional days.
• Overall stability depends on tumor cell type (ranges from days to weeks)
What cell types are compatible with the TME Kit matrix?
• Tumor cells (various types)
• Endothelial cells.
• Fibroblasts.
• PBMCs, lymphocytes, macrophages
What are the storage and handling requirements for the TME Kit components?
• Lyophilized components: Store at –20 °C.
• Plastics and buffers: Store at room temperature.
• Shelf life = 1 year
Can I combine the TME Kit with immunotherapy testing?
• Yes - likely. Immune cells can be integrated into the matrix or perfused through the chip.
• Immunotherapeutic agents can be added to perfusion to study effects on tumor–immune interactions.
What are typical assay readouts or endpoints when using the TME Kit?
• Immunofluorescence imaging (most common).
• Cytokine secretion analysis.
• Flow cytometry of circulating immune cells.
