Intestine-on-Chip

The Dynamic42 Intestine-on-Chip can be used to evaluate uptake and mechanistical features of pre-clinical drug candidates, chemicals and food additives or to study inflammatory and infectious diseases.

We use our perfusable Dynamic42 biochip platform to provide an innovative concept to setup in vivo-like microenvironments of the human intestine by reconstructing perfusable villus-like tissue structures.

Specifications

The Dynamic42 Intestine-on-Chip comprises two compartments: a vascular compartment and an intestinal compartment. The vasculature is formed by an endothelial lining and tissue-resident macrophages. The intestinal compartment comprises gut epithelial cells and migrated immune cells such as monocytes or dendritic cells.

The Dynamic42 Intestine-on-Chip can be operated with various cell sources. Please get in contact for further details.

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Endothelial Cell

macrophage-2

Macrophage

enterocyte-2

Enterocyte

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Goblet Cell

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Paneth Cell

dendritic-cell-2

Dendritic Cell

Two models can be operated in parallel on one Dynamic42 biochip.

The model has been tested in the following Dynamic42 biochips: BC001 and BC002.

Characteristics

Enhanced marker expression

Intense cell-cell communication

Physiologic biomechanical stimulation via flow

Secretory function

Immunocompetence

The Dynamic42 Intestine-on-Chip can be operated up to 14 days with high vitality, stable barrier function and stable marker expression.

Biomechanical stimulation ensures 3D in vivo-like tissue outgrowth with strong microvilli coverage of cell surfaces.

Slide 1

D42 Intestine-on-Chip: Brightfield image showing 14d cultured intestinal tissue structures. Intestinal epithelial cells form a villus-like tissue morphology with crypts inbetween. A three-dimensional tissue outgrowth is essential to enlarge the surface area for nutrient uptake and to provide tissue niches for immune cells and microbial organisms.

Slide 1

D42 Intestine-on-Chip: Endothelial cells and monocyte-derived macrophages comprise the vasculature of our Intestine-on-Chip model. Endothelial cells express von Willebrand factor (vWf) stained in green and CD31 stained in red. vWf plays a major role in platelet adhesion and in protective complex formation with factor VIII. CD31 mediates mechano-sensing of biomechanical stimuli and leukocyte adhesion to the vessel wall.

Slide 1

D42 Intestine-on-Chip: Endothelial cells and monocyte-derived macrophages comprise the vasculature of our Intestine-on-Chip model. Endothelial cells express CD31 in orange and tissue-resident macrophages are visualized by mannose receptor/CD206 staining in cyan.

Slide 1

D42 Intestine-on-Chip: Intestinal cells grow out into villus-like structures and differentiate under biomechanical stimulus through perfusion. Epithelial cells form a brush border and express villin in red. Villin is an F-actin bundling protein involved in the membrane extension of microvilli in the human intestine.

Slide 1

D42 Intestine-on-Chip: Intestinal cells grow out into villus-like structures and differentiate under biomechanical stimulus through perfusion. Epithelial cells express ZO-1 in cyan and E-cadherin in red. E-cadherin is a major component of intestinal adherens junctions.

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Applications

  • Toxicity profiling
  • Uptake / Transport studies
  • Host-Microbiome Interaction
  • Inflammatory Diseases (IBD)

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