Dynamic42 GmbH develops human, three-dimensional physiological organ models in a biochip. We are a contract research organization, and we are testing new drugs as well as novel dosage forms of drugs (i.e. nanoparticles). In addition, we are developing customized biochip-based assays and organ models (disease and infection models) for further studies.
Organ-on-Chip Contract Research Services
You can use our services for toxicology screenings, lead identification and optimization as well as in vitro model development. Our unique immune-competent organ models enable you to gain deeper understanding of human cellular biology in health and disease. It can help you to strengthen your pre-clinical data set, to perform target validation, to investigate molecular mechanisms and to prioritize further developments.
Our Services
Organ Model Options
/ Customization of standard organ models
/ Connected multi-organ models
/ New organ-on-chip model development
Applications
/ Compound testing
/ Pharmacokinetik/ Pharmacodynamik
/ Toxicology/ Metabolization
/ Disease modeling
/ Infection modeling
Dynamic42 Organ-on-Chip models
We offer services around our different Organ-on-Chip models:
How we work with you
Why work with us?
Human relevant data
Dynamic42 services are based on our unique, multicellular organ-on-chip technology integrating tissue-resident and circulating immune cell populations. Models can further contain a vasculature, microbiome or pathogens as needed.
Comprehensive and fast
Our motivated, well trained scientific team individually plans the scope of your study together with you, sets milestones and criteria for go and no-go decisions.
You decide how you work with us
Together we build up study plans to specifically address your scientific needs. This includes small pilot and feasibility studies, regular screenings, and extensive long-term projects.
Individually customizable organ models
We identify the optimal biological Organ-on-Chip setup for you. The great potential of the technology lies in the fact that individual biological and technical components can be easily modified. To achieve your goals, we make use of one principle: a modular cellular construction kit.
Comprehensive read-outs
/ NEW! TEER real-time monitoring
/ In Vitro Assay Development
/ Cell culture
/ Cytokine release assay
/ Hematoxylin & Eosin (H&E) Staining
/ Immunostaining
/ Inflammation Assays
/ Organotypic Culture
/ Oxidative Burst
/ Phenotype assays and reactive species assays
Unique biochip design & material for drug discovery
We provide services to design and fabricate customized biochips. Some applications or scientific questions need adjustments in biochip geometry or biochip materials. Our engineers provide technical expertise and support.
All our biochips are made of biocompatible, medical-grade material that shows very low adsorption rates for compounts of various kinds ensuing precise dosage prediction for preclinical testing of drugs.
ISO 9001 Certified
Dynamic 42 maintains a quality management system and is certified according to ISO 9001:2015. Learn more here.
Project examples
A three-organ-chip – liver, intestine, placenta for ADME & PBPK
In a collaboration with Bayer’s Consumer Health Division, MPSlabs (part of ESQlabs), and Placenta Lab of Jena University Hospital we have worked on an organ-on-chip platform and interactive computational software to understand whether small molecules can cross the blood-placenta-barrier in pregnant women. Within this project we have developed an interconnected three-organ model of the intestine, liver and placenta. In this study, the drug prednisone was administered into the gut model and then transported across the barrier and transferred via perfusion in the upper channel of each model. Metallization of the drug took place in the hepatocyte channel of the liver model, and potential transplacental transfer of the drug could be studied with the placenta-on-chip.
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A two-organ model – lung-liver for toxicology: A case study using Paraquat
In toxicology testing, multi-organ models help predict the often-complex metabolic pathways of drug compounds. We have developed a combined two-organ model consisting of lung and liver tissue and evaluated using Paraquat, a compound toxic to the lung.
Assessment of drug-induced liver injury – Trovafloxacin
Drug-induced liver injury (DILI) poses a significant risk to patient safety, often leading to drug withdrawals and considerable financial losses for the pharmaceutical industry. We have developed a liver model for assessment of DILI and evaluated it in case study with Trovafloxacin (TVX) and Levofloxacin (LVX).
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Assessment of drug-induced liver toxicity – Acetaminophen
Drug-induced liver injury (DILI) poses a significant risk to patient safety, often leading to drug withdrawals and considerable financial losses for the pharmaceutical industry. We have developed a liver model for assessment of DILI and evaluated it in case study with Acetaminophen
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Antibody-induced toxicities
Organ models can be used to predict immune-related antibody-induced toxicities. Immunomodulatory antibodies, emerged as key players in the treatment of cancer & inflammatory disease, need to be vetted carefully as they can cause severe side effects. We developed a model of the vasculature and evaluate with TGN1412, shown to cause life-threatening CD4 T cell/IL2-driven cytokine storm in clinical trials.
Modelling of the tumor microenvironment of pancreatic ductal adenocarcinoma on a spheroid-on-chip platform for drug discovery
Pancreatic ductal adenocarcinoma (PDAC) is characterized by a dense, fibrotic tumor microenvironment (TME) that interferes with drug delivery and helps evade the immune system. As a result, it is one of the most aggressive and deadly cancers, with a five-year survival rate of only 13%. Together with the research group of Prof. Dr. Nicole Teusch at the Heinrich Heine University, Düsseldorf, we have developed a biochip designed for PDAC-spheroids replicating key aspects of the TME. The model allows for dynamic drug administration and the infiltration of immune cells via a vasculature, providing a powerful tool for preclinical drug testing and reduction of animal testing
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Modeling invasive candidiasis in an intestine-on-chip model
We have developed a 3D intestine-on-chip model to investigate fungal-host interactions upon infection with Candida albicans and to investigate antifungal caspofungin treatment under clinically relevant conditions. By combining microbiological and image-based analyses, it was possible to quantify biological host alterations and to obtain novel in-depth insights into fungal microcolony morphology.
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Invasive aspergillosis-on-chip
In this study, we developed an Aspergillus fumigatus (A. fumigatus)infection model of on a human, in vitro model of the lung alveolus. In this invasive aspergillosis-on-chip model, A. fumigatus conidia are challenged by human primary monocyte-derived macrophages. Fungal hyphae growth parameters (e.g., length, branching level) and number of invasive hyphae are altered in the presence of macrophages. Antifungal drugs such as Caspofungin, when administered at clinically relevant concentrations also induced known morphological changes in A. fumigatus hyphae. This immunocompetent model offers a complex representation of lung tissue structure and function, offering a reliable platform for antifungal drug development and improving our understanding of fungal pathogenicity.
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Innovative model systems for the evaluation of immunomodulatory therapeutics
While preclinical studies have so far mainly investigated the basic toxicity of a new therapeutic agent on the (healthy) immune system, there is a lack of non-clinical models that accurately capture the individual interactions of the human immune system in the pathogenic state.
The EU consortium inSAVAR (Immune Safety Avatar: nonclinical mimicking of the immune system effects of immunomodulatory therapies) addresses this shortcoming with new concepts for testing immunomodulatory therapies. The aim is to improve existing and develop new model systems in order to:
/ Identify undesirable side effects of new therapies on the immune system
/ Develop new biomarkers for the diagnosis and prognosis of immune-mediated pharmacologies and toxicities
/ Explore toxicity mechanisms and the potential for their mitigation through therapeutic interventions in more detail.
/ The project thus aims to lay the foundations for new, Europe-wide standards in drug development.
The interdisciplinary consortium imSAVAR comprises 28 international partners from 11 nations under the scientific coordination of the Fraunhofer Institute for Cell Therapy and Immunology IZI (Leipzig, Germany) and Novartis (Basel, Switzerland).
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Antifungal therapy approaches with nanoparticle-based targeting of drugs
This research projects is embedded within the Athana alliance which brings together companies and research institutions from Thuringia that are leaders in pharmaceutical biotechnology and the infection and clinical research sector. The aim of this project is to develop functionalised nanoparticles that deliver drugs precisely to where they are needed in the body. The alliance aims to establish versatile platform technology for the use of target-guided nanoparticles for the treatment of infectious diseases. By targeting disease with medicine via functionalised nanoparticles, it will be possible to increase efficacy and reduce side effects due to the smaller quantities of drugs involved. With the help of a modular construction system, the innovative platform will permit the flexible production of therapeutic nanoparticles with different types of functionalities for the treatment of infectious diseases.
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Didn’t find what you were looking for?
If your research needs don’t fit with one of our standard services, let us know. We’ve got expert scientists who can work with you to create a custom collaboration project for your application of interest. Each of our organ-on-chip models and applications can be tailored to meet your research requirements. Just get in touch to let us know what you’re looking for, and our team will work with you to determine how we can help.