Liver-on-Chip

The Dynamic42 Liver-on-Chip can be used to evaluate toxicity and mechanistical features of pre-clinical drug candidates or to study inflammatory and infectious diseases.

We use our perfusable Dynamic42 biochip platform to provide an innovative concept to setup in vivo-like microenvironments of the human liver by reconstructing the smallest key functional tissue unit.

Specifications

The Dynamic42 Liver-on-Chip comprises two compartments: a vascular compartment and a hepatic compartment. The vasculature is formed by liver sinusoidal endothelial cells and tissue-resident macrophages serving as Kupffer cell surrogates. The hepatic compartment comprises hepatocytes and optionally hepatic stellate cells embedded in various kinds of matrices.

The Dynamic42 Liver-on-Chip can be operated with various cell sources. Please get in contact for further details.

endothelial-cell-2

Liver Sinusoidal Endothelial Cell

macrophage-2

Macrophage

hepatocyte-2

Hepatocyte

hepatic-stellate-cell-2

Hepatic Stellate Cell

Two models can be operated in parallel on one Dynamic42 biochip.

The model has been tested in the following Dynamic42 biochips: BC001 and BC002

Characteristics

Enhanced marker expression

Intense cell-cell communication

Physiologic biomechanical stimulation via flow

Secretory function

Immunocompetence

The Dynamic42 Liver-on-Chip can be operated up to 14 days with high vitality, stable secretory function and stable expression of transporters and CYP enzymes.

Embedding Vega-Lite
Embedding Vega-Lite
Embedding Vega-Lite
Embedding Vega-Lite
Slide 1

D42 Liver-on-Chip: Liver sinusoidal endothelial cells (LSECs) and tissue-resident macrophages comprise the vasculature of our Liver-on-Chip model. LSEC-specific marker FCGR2b/CD32b is shown in red and mannose receptor/CD206 on macrophages is shown in cyan.

Slide 1

D42 Liver-on-Chip: Hepatocytes and components of the extra-cellular matrix comprise the hepatic compartment of our Liver-on-Chip model. Hepatocytes express liver-specific marker asialglycoprotein receptor 1 (ASGPR-1) in red. It plays an important role in targeted drug delivery as well as in autoimmune hepatitis, and it can acts as entry point for infections in the human liver.

Slide 1

D42 Liver-on-Chip: Hepatocytes and components of the extra-cellular matrix comprise the hepatic compartment of our Liver-on-Chip model. Hepatocytes express cytochrome P450 enzyme 3A4 (CYP3A4) in orange. CYP3A4 is mainly located in the human liver and is heavily involved in the metabolism of ≥ 75 % of drugs that humans get administered.

Slide 1

D42 Liver-on-Chip: Hepatocytes and components of the extra-cellular matrix comprise the hepatic compartment of our Liver-on-Chip model. Hepatocytes express cytochrome P450 enzyme 3A4 (CYP3A4) in orange and liver-specific marker asialglycoprotein receptor 1 (ASGPR-1) in red. Both are shown as overlay image.

Slide 1

D42 Liver-on-Chip: Hepatocytes and components of the extra-cellular matrix comprise the hepatic compartment of our Liver-on-Chip model. Hepatocytes express glutathione-S-transferanse (GST) stained in cyan. GST plays a major role in the detoxification of xenobiotics. Additionally, ASGPR-1 is stained in red. Both are shown as overlay image.

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Applications

  • Toxicity profiling
  • Uptake / Transport studies
  • Antibody recycling
  • Cholestasis
  • Infectious disease modelling

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